These somatic depolarizations enhanced somatic excitability by increasing the probability of action potential generation. Photolytic uncaging of p-hydroxyphenacyl (pHP) GABA demonstrates backpropagation of GABA AR-mediated depolarizations from MNTB axon terminals to the soma, some hundreds of microns away. Postembedding immunogold labeling and electron microscopy provide evidence that GABA ARs are localized at MNTB axon terminals. ![]() This spillover excitation generates patterns of staggered neurotransmitter release from different MNTB axons resulting in characteristic “doublet” postsynaptic currents in LSO neurons. Here we use electrophysiological recordings in brainstem slices from P3-P21 mice to demonstrate that GABA release evoked from MNTB axons can spill over to neighboring MNTB axons and cause excitation by activating GABA AR. ![]() The role for this neurotransmitter cotransmission is poorly understood. Synapses from neurons of the medial nucleus of the trapezoid body (MNTB) onto neurons of the lateral superior olive (LSO) in the auditory brainstem are glycinergic in maturity, but also GABAergic and glutamatergic in development.
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